NEPA21 高效基因電轉(zhuǎn)染儀------適用于體外(In Vitro)和活體(In Vivo)類(lèi)器官轉(zhuǎn)染
讓轉(zhuǎn)染更簡(jiǎn)單、更Free
NEPA GENE公司專(zhuān)業(yè)研發(fā)、生產(chǎn)細(xì)胞電轉(zhuǎn)染儀及電融合儀等,其生產(chǎn)CUY21系列電轉(zhuǎn)染儀在研究領(lǐng)域中久負(fù)盛名,已被數(shù)百篇文獻(xiàn)引用,其中不乏高水平雜志的文章,如Nature、Cell、PNAS、Genes & Dvelopment等。
2011年,NEPA GENE推出新一代多功能型NEPA21高效基因轉(zhuǎn)染系統(tǒng)。與傳統(tǒng)的電轉(zhuǎn)儀相比,NEPA21采用全新設(shè)計(jì)的電轉(zhuǎn)程序,特別適用于難轉(zhuǎn)染細(xì)胞、離體組織或動(dòng)物活體的轉(zhuǎn)染。配合的電壓衰減(Voltage Decay)設(shè)計(jì),NEPA21可在獲得高轉(zhuǎn)染效率的同時(shí),提高細(xì)胞存活率。
主要特點(diǎn):
全新設(shè)計(jì)的電轉(zhuǎn)程序,
特別適用于難轉(zhuǎn)染細(xì)胞、離體組織或動(dòng)物活體的轉(zhuǎn)染,
高轉(zhuǎn)染效率、高細(xì)胞存活率
電轉(zhuǎn)程序各項(xiàng)參數(shù)可見(jiàn)、可調(diào),適用性廣
不需要特殊的轉(zhuǎn)染試劑盒輔助,運(yùn)維成本低,胚胎基因編輯
NEPA21 高效基因電轉(zhuǎn)染儀應(yīng)用范圍:
? 懸浮轉(zhuǎn)染
適用范圍:原代細(xì)胞、干細(xì)胞、以及各種難轉(zhuǎn)染細(xì)胞(如免疫細(xì)胞、血液細(xì)胞等)
? 貼壁轉(zhuǎn)染
適用范圍:可以直接對(duì)貼壁細(xì)胞進(jìn)行轉(zhuǎn)染,省去了細(xì)胞消化、再貼壁的步驟(對(duì)提高某些種類(lèi)細(xì)胞的存活率十分重要)!
? 離體組織轉(zhuǎn)染
適用范圍:組織切片、腦切片、器官、胚胎等
? 活體轉(zhuǎn)染
適用范圍:大腦、視網(wǎng)膜、肌肉、皮膚、肝臟、腎臟、睪丸等
部分參考文獻(xiàn):
Barnabe-Heider et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods, 2008 Feb;5(2):189-96.
Shibata MA, et al. Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model. Cancer Gene Ther. 2008 Dec;15(12):776-86.
Limura T and Pourquie O. Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression. Nature, 2006 Aug 3;442(7102):568-71.
Sanada K and Tsai LH. G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors. Cell, 2005 Jul 15;122(1):119-31
Ladher RK et al. FGF8 initiates inner ear induction in chick and mouse. Genes and Development, 2005 Mar 1;19(5):603-13.